Increased Expression of RhoA in Epithelium and Smooth Muscle of Obese Mouse Models: Implications for Isoprenoid Control of Airway Smooth Muscle and Fibroblasts
Figure 7
U18666a increases RhoA expression in ASM and decreases RhoA expression in NHLF. RhoA expression by western blot is increased in ASM after 72 hours of treatment by U18666a (5 μg/mL) compared to untreated cells (NT) but is decreased in NHLF cells, which is consistent with the differential effects of U18666a on cell proliferation in these cell types. (a) Representative blot of ASM. (b) Densitometry shown as mean of 3 experiments, each run in duplicate or triplicate; RhoA density is normalized to actin. (c) Representative blot of NHLF. (d) Densitometry shown as mean of 3 experiments, each run in duplicate or triplicate; RhoA density is normalized to actin. Means are compared by the Student’s -test, .
