Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78/CXCL5 and Reduces Neutrophil Migration

Figure 3

FCS and AEBSF block the effect of HDM extract. (a) Effect of HDM extract (2 and 10 μg/mL) on ENA-78 levels in the presence of 5% FCS. ENA78/CXCL5 levels were measured in different HDM containing CM preparations after 24 h (left panel) and 72 h (right panel). (b) Effect of FCS (5%) on HDM extract-induced reduction of ENA-78/CXCL5 levels (𝑛=5; ^∗∗∗𝑃<0.001 comparing HDM extract stimulated CM to unstimulated CM). (c) Degradation of recombinant ENA-78/CXCL5 by HDM extract is inhibited by the addition of the serine proteases inhibitor AEBSF or FCS (𝑛=3, measured as duplicates; bars represent the mean ± SEM, ^∗∗∗𝑃<0.001 relative to the rescue effect of AEBSF on HDM extract-induced ENA-78/CXCL5 degradation).

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