The proteolytic effects of tryptase on cytokine-induced CXCL10 production by asthmatic (A) and non-asthmatic (NA) ASM cells. Confluent serum-deprived ASM cells were stimulated with (a) IL-1, (b) TNF, (c) IFN, or (d) cytomixfor 30 minutes prior to and during treatment with tryptase, heat-inactivated tryptase (HI), or its vehicle (veh) for 24 h. Leupeptin (Leu) was added to some cell cultures 3 h after the cytokines. CXCL10 in the culture supernatants was detected using ELISA. compared with the vehicle control, ANOVA.
