The effects of human lung mast cell products released early after activation on asthmatic (A) and non-asthmatic (NA) ASM cell CXCL10 production. Mast cells (MC) were isolated from human lung and immediately activated with IgE/anti-IgE in culture medium (DMEM + 10% FBS) and the culture supernatants collected after 2 hours (2 h MC SN). The MC SN were added at 20% and 40% v/v to ASM cells stimulated with IFN and CXCL10 levels were quantified after 48 hours stimulation using ELISA. compared with the DMEM + 10% FBS control (a) or FBS-IFN control ((b) and (c)), ANOVA.
