Human Lung Mast Cell Products Regulate Airway Smooth Muscle CXCL10 Levels

Figure 6

The effects of human lung mast cell products released later after activation (2–24 h) on asthmatic (A) and non-asthmatic (NA) ASM cell CXCL10 production. Mast cells (MC) were isolated from human lung and immediately activated with IgE/anti-IgE in culture medium (DMEM + 10% FBS). After 2 hours the culture supernatants were collected (2 h MC SN) and replaced by fresh medium. Further supernatants were collected at 24 hours (24 h MC SN). The 24 h MC SN were added at 20% and 40% v/v to IFN𝛾-stimulated ASM cells and after 48 hours CXCL10 levels were quantified using ELISA (a). TNF-𝛼 levels in the 2 h and 24 h MC SN were quantified using ELISA (b) and the effects of these low concentrations of TNF-𝛼 on IFN𝛾-induced CXCL10 production by ASM cells in DMEM + 10% FBS quantified (c). ^∗𝑃<0.05 compared with the DMEM + 10% FBS control ((a) and (c)), ANOVA.

(a)  

(b)

(c)