Cloning and Expression of Ama r 1, as a Novel Allergen of Amaranthus retroflexus Pollen

Figure 1

Comparison of the A. retroflexus Ole e 1-like protein (Ama r 1) amino acid sequence with allergenic Ole e 1-like protein from other plants. Chenopodium album (Che a 1, G8LGR0.1), Crocus sativus (Cro s 1, XP004143635.1), Salsola kali (Sal k 5, ADK22842.1), Olea europaea (Ole e 1, P19963.2), Fraxinus excelsior (Fra e 1, AAQ83588.1), Syringa vulgaris (Syr v 1, S43243), and Ligustrum vulgare (Lig v 1, O82015.2). The amino acid sequence identity and the similarity of Ama r 1 (KR870437) to other members of the Ole e 1-like family are shown in Table 2. The top line indicates the location of secondary structures that are created by PSIPRED protein sequence analysis (http://bioinf.cs.ucl.ac.uk/psipred/). The cylinder, arrows, and black line correspond to alpha-helices, beta-strands, and coil structure, respectively.