Comparative Study on Biochemical Properties and Antioxidative Activity of Cuttlefish (Sepia officinalis) Protein Hydrolysates Produced by Alcalase and Bacillus licheniformis NH1 Proteases

Table 6

Amino acid composition (% mole) of CPHs using Alcalase and NH1 proteases. Values shown are mean values of three measurements.

Amino acid^a	Alcalase-CPH	NH1-CPH
Aspartic acid	6.01	6.14
Threonine^c	2.10	2.66
Serine	9.14	9.80
Glutamic acid	11.33	9.54
Glycine	3.05	3.11
Alanine	3.65	3.60
Valine^c	6.01	6.89
Methionine^c	2.95	3.01
Isoleucine^c	3.62	3.81
Leucine^c	8.78	8.90
Tyrosine	5.80	5.72
Phenylalanine^c	4.01	3.99
Histidine^c	11.61	11.23
Lysine^c	9.77	9.55
Arginine	11.71	11.57
Cysteine	0.17	0.15
Proline	0.29	0.33
TAA^d	100	100
THAA^d	32.36	33.64
TEAA/TAA%	48.85	50.04

^aThe aspartic and glutamic acid contents include, respectively, asparagines and glutamine.
^bUndigested cuttlefish muscle protein.
^cEssential amino acids.
^dTAA ≡ total amino acids; THAA ≡ total hydrophobic amino acids; TEAA ≡ total essential amino acids.