Research Article
Simple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis
Table 1
Purification of trypsin from Pacific cod.
| | Purification stages | Protein (mg) | Total activity (U) | Specific activity (U/mg) | Purity (fold) | Yield (%) |
| | Activated crude extract | 503 | 5,281 | 10.5 | 1.0 | 100 | | 80% (NH4)2SO4 fraction | 424 | 5,217 | 12.3 | 1.2 | 99 | | Gel filtration | 17.8 | 3,400 | 191 | 18 | 64 |
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