Simple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis

Table 3

Kinetic parameters for Pacific cod trypsin-catalyzed hydrolysis.

Substrate	Parameter	PcT	BT	PcT/BT
BAPA	𝐾𝑚 (mM)	0.037	0.57	0.063
	𝐾cat (sec^−1)	3.7	1.7	2.2
	𝐾cat/𝐾𝑚 (mM^−1s^−1)	100	2.9	35
TAME	𝐾𝑚 (mM)	0.0015	0.021	0.074
	𝐾cat (sec^−1)	108	86	1.3
	𝐾cat/𝐾𝑚 (mM^−1s^−1)	71,206	4,160	17
N-Boc-L-Ala-OpAm (4a)	𝐾𝑚 (mM)	0.0015	0.021	0.071
	𝐾cat (sec^−1)	2.0	1.2	1.6
	𝐾cat/𝐾𝑚 (mM^−1s^−1)	1,449	619	2.3

PcT, Pacific cod trypsin; BT, Bovine trypsin.