382107.fig.002a
(a)
382107.fig.002b
(b)
382107.fig.002c
(c)
Figure 2: Representation of NF-H and NF-M mutants. (a) Incorporation of a C-terminal myc tag results in loss of 612 amino acids including all KSP repeat motifs. (b) Incorporation of a C-terminal myc tag results in loss of 426 amino acids including 7 KSP repeat motifs. (c) Site-directed mutagenesis was utilized to mutate all KSP serine residues to alanine thereby preventing KSP serine phosphorylation without deletion of the remaining amino acids. The approximate location of each KSP repeat is identified.