Neurofilament Phosphorylation during Development and Disease: Which Came First, the Phosphorylation or the Accumulation?

Figure 3

Relative positions of CMT2E-linked NF-L mutations and NF-L head domain phosphorylation sites. (A) Identity and location of all identified CMT2E-linked NF-L mutations. (B) Aligned protein sequences of human, bovine, orangutan, murine, and rat NF-L N-terminal head domains. Identified serine phosphorylation sites within bovine NF-L are indicated along the consensus sequence. Shaded boxes indicate conserved sequence variations, and empty boxes identify nonconserved sequence variations. An X in the consensus sequence identifies amino acid positions that lack an overall consensus between the five species. Sequence homology is indicated by percent identity. CMT2E-linked head domain mutations NF-L^P8R and NF-L^P22S are identified with an arrow with the corresponding amino acid substitution. These two mutations are located adjacent to phosphorylation sites. Notice that all but one (ser⁶⁹) bovine phosphorylation site is conserved between species. Sequence accession numbers used to generate this figure are as follows: Human NP_006149, Bovine NP_776546, Orangutan NP_001126494, Mouse NP_035040, and Rat NP_113971.