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Figure 3: Relative positions of CMT2E-linked NF-L mutations and NF-L head domain phosphorylation sites. (A) Identity and location of all identified CMT2E-linked NF-L mutations. (B) Aligned protein sequences of human, bovine, orangutan, murine, and rat NF-L N-terminal head domains. Identified serine phosphorylation sites within bovine NF-L are indicated along the consensus sequence. Shaded boxes indicate conserved sequence variations, and empty boxes identify nonconserved sequence variations. An X in the consensus sequence identifies amino acid positions that lack an overall consensus between the five species. Sequence homology is indicated by percent identity. CMT2E-linked head domain mutations and are identified with an arrow with the corresponding amino acid substitution. These two mutations are located adjacent to phosphorylation sites. Notice that all but one (ser69) bovine phosphorylation site is conserved between species. Sequence accession numbers used to generate this figure are as follows: Human NP_006149, Bovine NP_776546, Orangutan NP_001126494, Mouse NP_035040, and Rat NP_113971.