575180.fig.002a
(a)
575180.fig.002b
(b)
575180.fig.002c
(c)
Figure 2: Direct Fluorescence-Binding Assays of NSP4112-140 with Caveolin-1 Peptides in the presence of 1 μM SUV model membranes. Direct binding based on dequenching of Cy3 fluorescence emission. (a–c) Plots of maximal fluorescence emission (measured at 565 nm upon excitation at 550 nm) for NSP4112-140 in the presence of 1 uM SUV and either 25 nM Cy3-N-Cav2-20 (a), 50 nM Cy3-Cav19-40 (b), or 100 nM Cy3-C-Cav161-178 (c). Insets: linear plots of the binding curve of NSP4112-140.