Elucidation of the Rotavirus NSP4-Caveolin-1 and -Cholesterol Interactions Using Synthetic Peptides

Figure 2

Direct Fluorescence-Binding Assays of NSP4_112-140 with Caveolin-1 Peptides in the presence of 1 μM SUV model membranes. Direct binding based on dequenching of Cy3 fluorescence emission. (a–c) Plots of maximal fluorescence emission (measured at 565 nm upon excitation at 550 nm) for NSP4_112-140 in the presence of 1 uM SUV and either 25 nM Cy3-N-Cav_2-20 (a), 50 nM Cy3-Cav_19-40 (b), or 100 nM Cy3-C-Cav_161-178 (c). Insets: linear plots of the binding curve of NSP4_112-140.

(a)

(b)

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