Table 5: Treatment with cholesterol-altering drugsa.

InhibitorActionResults

FillipinCholesterol-binding; disrupts caveolae and caveolar membrane transport.Disrupted NSP4 transport
NystatinCholesterol-binding; disrupts caveolae and caveolar membrane transport.Disrupted NSP4 transport
ChlorpromazineInhibits clathrin-dependent endocytosis and coated pit processes.Normal NSP4 transport to the PM.
NocodazoleDisrupts microtubule polymerizationDisrupted NSP4 transport

aEach inhibitor was reacted with RV-infected MDCK cells. The cells were washed, stained for NSP4 (anti-NSP4150-175) and a fluorescent secondary antibody (rabbit anti-IgG-FITC), and the intracellular location compared to untreated, infected cells. Chlorpromazine and nocodazole were included as controls.