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Journal of Amino Acids
Volume 2012 (2012), Article ID 650840, 6 pages
http://dx.doi.org/10.1155/2012/650840
Research Article

Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy

1Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA
2Department of High Technologies, The International Solomon University, 1b Sholudenko Street, Kyiv 01135, Ukraine
3Department of Application Services, Infopulse Ukraine LLC, 24 Polyova Street, Kyiv 03056, Ukraine

Received 21 June 2011; Accepted 13 August 2011

Academic Editor: Kunchithapadam Swaminathan

Copyright © 2012 Nina A. Filenko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well. To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging. The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template. Di-nucleosomes in close proximity were observed, but their population was low. The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes. The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed.