Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance

Figure 5

Examples of amine- and thiol-based immobilization methods using sensor chips coated with a carboxymethyl-modified dextran layer. (a): NHS/EDC-coupling of amine functionalized ligands, (b): immobilization of aldehyde funtionalized ligands using reductive ammination, (c): disulphide exchange, (d): ligation of thiol derivatives to maleimides, (e): native chemical ligation.