Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance

Figure 9

Addition of antisense RNA to the chimeric oligonucleotide consisting of DNA and RNA, results in the hybridization of the antisense strand and its complementary RNA sequence. RNase H only recognizes RNA-DNA heteroduplexes and cleaves the corresponding RNA strand. The resulting chimeric fragments end up in the next flow cell (flow cell 2). Due to the complementarity between the immobilized single stranded DNA in flow cell 2 and the DNA of the chimeric oligonucleotide fragments, both strands hybridize and induce a response in flow cell 2.