Role of Linkers between Zinc Fingers in Spacing Recognition by Plant TFIIIA-Type Zinc-Finger Proteins

Figure 5

Preference for the sequences around AGT core sites. ZPT2-14ZF was tested for binding to a set of probes having one-base mutations outside the AGT core sites. Fractions of bound DNA are shown as relative values to the bound fraction for AGT-10-AGT, with representative electrophoretic patterns above them. The data are averages of four experiments, with bars representing standard deviations. The probes are essentially the same as AGT-10-AGT in Figure 3 except for the mutated bases as indicated by lowercase letters.