Peptide Mass Fingerprinting and N-Terminal Amino Acid Sequencing of Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham.

Table 3

Comparison of N-terminal sequences of Nivulian-II with other known cysteine proteases.

Source

Enzyme

Sequence*

Identities

Positives

Euphorbia nivulia

Nivulian-II

D

F

P

P

N

T

C

C

C

I

C

C

—

—

Opisthorchis viverrini

Cathepsin F

M

R

P

F

V

C

C

V

L

V

T

T

2/12 (16.66)

3/12 (25.00)

Streptococcus pyogenenes

IdeS

K

S

C

D

K

T

H

T

C

P

P

C

3/12 (25.00)

3/12 (25.00)

Zingiber officinale

GP-I

D

V

L

P

D

S

I

D

W

R

E

K

2/12 (16.66)

3/12 (25.00)

Zingiber officinale

GP-II

D

D

L

P

D

S

I

D

W

R

E

N

2/12 (16.66)

3/12 (25.00)

Pineapple stem

Bromelain

—

A

V

P

Q

S

I

D

W

R

D

Y

1/12 (08.33)

2/12 (16.66)

Rat liver

Cathepsin J

D

T

P

A

N

E

T

Y

P

D

L

L

3/12 (25.00)

3/12 (25.00)

∗Shared amino acids as bold.