Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP
Figure 3
SDS-PAAG-electrophoresis of the recombinant SPS preparation. From left to right: (a) Raw extract, supernatant; highly-purified SPS ( kDa); raw extract, pellet; a protein ladder of markers (SeeBlue Plus Pre-Stained Standard; Invitrogen, Cat # LC5925), (b)—a highly-purified preparation of SPS after DEAE-Sephadex A-50, markers. Stained with Brilliant Blue G.