Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP

Table 1

Purification of the prokaryotic SPS from the E. coli DH5α-T1 strain containing a plasmid with a cloned selD gene from the BL21-Gold (DE3) strain.

Expression	Purification index
	Cell quantity, raw weight	Protein amount in the raw extract, mg	Amount of the protein loaded onto a column with DEAE-Sephadex, mg	Amount of the protein eluted from the column, mg
Without an inductor	6	75	61	7.8
With an inductor (IPTG)	6	185	150	28