L-Phenylalanine Transport in Saccharomyces cerevisiae: Participation of GAP1, BAP2, and AGP1
Figure 1
Phenylalanine uptake into Y294 wild type and mutant yeast strains inactivated in GAP1, BAP2, and AGP1. The strains were grown in MP (black bars) or MA (shaded bars) media containing leucine, histidine, tryptophan, and uracil. The L-[14C]-phenylalanine uptake was assayed as described in Section 2. Phenylalanine uptake was determined at an amino acid concentration of 20 μM ((a) and (c)); phenylalanine uptake was determined at amino acid concentration of 1.0 mM ((b) and (d)).
