A Fully Automated Sequential-Injection Analyser for Dual Electrogenerated Chemiluminescence/Amperometric Detection

Economou, Anastasios; Nika, Maria

doi:https://doi.org/10.1155/JAMMC/2006/67571

Journal of Analytical Methods in Chemistry

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Volume 2006 | Article ID 067571 | https://doi.org/10.1155/JAMMC/2006/67571

A Fully Automated Sequential-Injection Analyser for Dual Electrogenerated Chemiluminescence/Amperometric Detection

Anastasios Economou¹and Maria Nika¹

Received18 Oct 2005

Revised29 Dec 2005

Accepted16 Jan 2006

Published25 May 2006

Abstract

This work describes the development of a dedicated, fully automated sequential-injection analysis (SIA) apparatus suitable for simultaneous electrogenerated chemiluminescence (ECL) and amperometric detection. The instrument is composed of a peristaltic pump, a multiposition selection valve, a home-made potentiostat, a thin-layer electrochemical/optical flow-through cell, and a light detector. Control of the experimental sequence and simultaneous data acquisition of the light and the current intensities were performed in LabVIEW6.1. The CL reagents and the sample were first aspirated as distinct zones into the holding coil of the analyser and, then, delivered to the cell; during their travel, the individual zones mixed and the ECL reaction was initiated as soon as the mixed regents reached the cell. The utility of the analyser was demonstrated for the detection of oxalate and H2O2 based on their ECL reactions with Ru(bpy)32 and luminol, respectively.

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Copyright

Copyright © 2006 Anastasios Economou and Maria Nika. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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