Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics

Figure 6

Affinity tests on the representatives of aptamer groups evolved from Capture-SELEX. Affinity tests were performed similar to the Capture-SELEX procedure, and ssDNA was eluted by different concentrations of kanamycin A in selection buffer. Data was fitted by the model of one site direct binding using a rectangular hyperbola for the saturation curve (OriginLab Corporation, OriginPro 8G SR2).