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Journal of Analytical Methods in Chemistry
Volume 2012 (2012), Article ID 450716, 10 pages
http://dx.doi.org/10.1155/2012/450716
Review Article

Flow-Based Systems for Rapid and High-Precision Enzyme Kinetics Studies

1Department of Chemistry, Xavier University, 3800 Victory Parkway, Cincinnati, OH 45207, USA
2Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
3Center of Excellence in Innovation for Analytical Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand

Received 30 November 2011; Accepted 28 January 2012

Academic Editor: Sibel A. Ozkan

Copyright © 2012 Supaporn Kradtap Hartwell and Kate Grudpan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into and out of the detector. By using the automatic flow-based analytical systems, enzyme kinetics studies can be carried out at real-time initial rate avoiding the potential errors inherent in manual operation. Flow-based systems have been developed to provide rapid, low-volume, and high-precision analyses that effectively replace the many tedious and high volume requirements of conventional wet chemistry analyses. This article presents various arrangements of flow-based techniques and their potential use in future enzyme kinetics applications.