Journal of Analytical Methods in Chemistry

Research Article

RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma

Table 1

Imprecision and inaccuracy results of validation.
Spiked conc. (μg/L)Conc. found mean ± SD (μg/L)CV (%)Accuracy (%)
Intraday imprecision and inaccuracy ( 𝑛 = 1 8 )
150 1 5 1 . 8 ± 6 . 0 0 4.0101.2
450 4 4 2 . 3 ± 1 9 . 5 1 4.498.3
800 7 8 0 . 6 ± 2 5 . 3 0 3.297.6
Interbatch imprecision and inaccuracy ( 𝑛 = 1 2 )
150 1 5 3 . 9 ± 5 . 2 0 3.4102.6
450 4 3 0 . 5 ± 1 6 . 7 0 3.995.7
800 8 1 0 . 6 ± 3 0 . 3 3.7101.3
Interday imprecision and inaccuracy ( 𝑛 = 1 2 )
150.0 1 5 6 . 4 ± 8 . 0 4 5.1104.3
450.0 4 3 5 . 2 ± 1 7 . 9 0 4.196.7
800.0 7 5 6 . 2 ± 2 0 . 4 5 2.794.5
Analyst to analyst imprecision and inaccuracy ( 𝑛 = 1 8 )
150.0 1 6 0 . 0 ± 1 0 . 3 0 6.4106.7
450.0 4 5 8 . 3 ± 9 . 8 0 2.1101.8
800.0 7 8 0 . 2 ± 2 4 . 9 0 3.297.5
Global imprecision and inaccuracy ( 𝑛 = 3 0 )
150.0 1 5 2 . 9 ± 6 . 6 0 4.3101.9
450.0 4 3 6 . 4 ± 2 2 . 0 5 5.197.0
800.0 7 9 5 . 6 ± 3 6 . 5 9 4.699.5