Table 1: Results of validation parameters of the responses and the regression equations obtained by the proposed methods. 
 Parameters  Method (A)  Method (B) 
 Slope^{a}  0.9706  0.1086  Intercept^{a}  0.0365  0.0684  Correlation coefficient  0.9998  0.9994  Concentration range  1–10 μg/mL  0.5–5 μg/band  Average accuracy (%)  99.3  99.7  S.D.  0.7  0.7  R.S.D.%  0.7  0.7  Specificity ± R.S.D.  99.4 ± 1.3  100.1 ± 1.2  Repeatability^{b} % ±R.S.D.  100.1 ± 0.1  99.8 ± 0.6  Intermediate precision^{c} % ±R.S.D.  99.8 ± 0.2  99.4 ± 0.2 


^{
a}Results of five determinations. ^{
b}. ^{
c} = 3 × 3.
