Table 1: Results of validation parameters of the responses and the regression equations obtained by the proposed methods.

ParametersMethod (A)Method (B)

Slopea0.97060.1086
Intercepta0.03650.0684
Correlation coefficient0.99980.9994
Concentration range1–10 μg/mL0.5–5 μg/band
Average accuracy (%)99.399.7
S.D.0.70.7
R.S.D.%0.70.7
Specificity ± R.S.D.99.4 ± 1.3100.1 ± 1.2
Repeatabilityb %  ±R.S.D.100.1 ± 0.199.8 ± 0.6
Intermediate precisionc %  ±R.S.D.99.8 ± 0.299.4 ± 0.2

aResults of five determinations.
b 𝑛 = 3 × 3 .
c 𝑛 = 3 × 3.