A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study

Table 1

Matrix effects and extraction recovery of aesculin and the internal standard in rat plasma.

Analytes	Concentration (ng/mL)	Matrix effects; (%, 𝑛=6)	CV (%)	Extraction recovery; (%, 𝑛=5)	CV (%)
Aesculin	40.0	98.1 ± 5.6	5.7	97.6 ± 7.4	7.6
	500.0	106.4 ± 3.8	3.6	95.8 ± 6.6	6.9
	1200.0	94.2 ± 6.9	7.3	105.3 ± 4.1	3.9
IS	10.0	96.2 ± 3.3	3.4	97.5 ± 5.8	5.9
	10.0	100.6 ± 5.2	5.2	104.7 ± 4.8	4.6
	10.0	104.2 ± 4.1	3.9	98.2 ± 3.9	4.0