A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study

Table 3

Intraday (𝑛=5) and interday (𝑛=5) precision and accuracy for assay of aesculin in rat plasma.

Concentration (ng/mL)	Intraday (𝑛=5)			Interday (𝑛=5)
	Mean ± SD (ng/mL)	RSD (%)	Accuracy (%)	Mean ± SD (ng/mL)	RSD (%)	Accuracy (%)
40.0	40.2 ± 1.4	3.5	100.5	38.5 ± 1.2	3.1	96.3
500.0	487.5 ± 15.2	3.1	97.5	490.7 ± 11.6	2.4	98.1
1200.0	1226.0 ± 86.3	7.0	102.2	1217.0 ± 75.8	6.2	101.4