Optimization of IC Separation Based on Isocratic-to-Gradient Retention Modeling in Combination with Sequential Searching or Evolutionary Algorithm

Table 1

Parameters of isocratic retention models described by (1). The eluent concentration is expressed as mM.

	50% peak height at fronting side				Peak maximum				50% peak height at tailing side
	𝑎0	𝑎1	𝑎2	𝑅^2	𝑎0	𝑎1	𝑎2	𝑅^2	𝑎0	𝑎1	𝑎2	𝑅^2
Arabitol	−0.201	−0.009	−0.031	0.9580	−0.188	−0.007	−0.030	0.9723	−0.153	−0.020	−0.027	0.9689
Cellobiose	1.808	0.127	−0.286	0.9999	1.814	0.125	−0.285	0.9999	1.820	0.126	−0.285	0.9999
Fructose	1.224	0.128	−0.261	0.9999	1.230	0.128	−0.261	0.9999	1.237	0.124	−0.259	1.0000
Fucose	0.353	0.282	0.234	1.0000	0.363	0.278	−0.231	1.0000	0.374	0.274	−0.228	1.0000
Lactulose	1.552	0.152	−0.291	1.0000	1.558	0.152	−0.291	1.0000	1.566	0.149	−0.289	1.0000
Melibiose	1.298	0.205	−0.288	1.0000	1.304	0.204	−0.288	1.0000	1.311	0.201	−0.286	1.0000
N-Acetyl-D-glucosamine	1.168	0.036	−0.243	0.9998	1.177	0.028	−0.239	0.9998	1.185	0.028	−0.238	0.9998
Raffinose	1.327	0.346	−0.262	0.9989	1.335	0.345	−0.261	0.9989	1.343	0.341	−0.260	0.9989