Fluorimetric Immunoassay for Multianalysis of Aflatoxins

Table 4

Recovery studies of real milk sample fortified with different aflatoxins.

Milk samples	Aflatoxin type	Added (pg/mL)	Found (pg/mL)	R.S.D.%	R.E.%	Recovery%
CRM-BD282	AFM1	50	50	0	0	100
CRM-BD282 + S1*	AFM1	50	48.6	47 ± 8	−2.8	97.2
CRM-BD282 + S2*	AFM1	50	46	46.2 ± 1.08	−8	92
CRM-BD282	AFM2	50	45.5	45 ± 0.55	−9	91
CRM-BD282 + S1*	AFM2	50	44.8	44.8 ± 4.44	−10.4	89.6
CRM-BD282 + S2*	AFM2	50	40.2	41 ± 3.75	−19.6	80.4
CRM-BD282	AFB1	50	40.5	40 ± 5	−19	81
CRM-BD282 + S1*	AFB1	50	50.4	50.2 ± 4.96	0.8	100.8
CRM-BD282 + S2*	AFB1	50	46.8	46 ± 4.34	−6.4	93.6
CRM-BD282	AFG1	50	50.2	50 ± 3.2	0.4	100.4
CRM-BD282 + S1*	AFG1	50	46.3	46 ± 1.76	−7.4	92.6
CRM-BD282 + S2*	AFG1	50	46.8	46 ± 4.34	−6.4	93.6

S1*, S2*: real milk samples.