Rapid Enzymatic Method for Pectin Methyl Esters Determination

Figure 4

PH influence on the calibration curve. a: pH 7.0: Δ𝐴Δ𝑡⁻¹(mAs⁻¹)=(0.44±0.02)×𝐶(mM)+(0.2±0.1), 𝑟=0.999, b: pH 7.5: Δ𝐴Δ𝑡⁻¹(mAs⁻¹)=(0.433±0.009)×𝐶(mM)+(0.15±0.05), 𝑟=0.9998, and c: pH 6.5: Δ𝐴Δ𝑡⁻¹(mAs⁻¹)=(0.44±0.02)×𝐶(mM)+(0.0±0.1), 𝑟=0.9991.