Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS⁺ System

Table 2

Analysis of gradient elution conditions with RP-HPLC.

Instrument	Condition
Reverse phase column	RS-Tech Optimapak C18 (4.6 × 250 mm, 5 m)
Oven temp. (°C)	40
Flow rate (mL/min)	1.0
Mobile phase (%)	A: 0.1% TFA in water, B: acetonitrile
UV absorbance (nm)	210, 254, 280, 320
Time (min)	Solvent composition (%)
	0.1% TFA in water; (A)	Acetonitrile; (B)
0	90	10
50	60	40
60	60	40
70	90	10