Quantification of Neurotransmitters in Mouse Brain Tissue by Using Liquid Chromatography Coupled Electrospray Tandem Mass Spectrometry

Table 2

Determination of neurotransmitters by LC-MS/MS: validation results on precision and accuracy.

Chemical	Intraday precision^a			Interday precision^b			Accuracy (%)			RE (%)
	Low	Mid	High	Low	Mid	High	Low	Mid	High	Low	Mid	High
BH4	1.18	1.09	0.42	4.28	1.78	0.59	102.90	99.72	97.48	2.90	0.28	2.53
Dopamine	1.15	1.26	0.73	3.98	1.90	0.86	103.07	99.12	97.80	3.07	0.88	2.21
Serotonin	8.30	8.12	8.20	8.58	9.44	9.23	95.57	108.46	96.15	4.43	8.46	3.85
Norepinephrine	8.21	6.95	4.50	10.17	7.28	4.93	104.90	103.06	98.75	4.90	3.06	1.26
Epinephrine	6.22	4.51	2.45	6.88	7.50	2.57	93.00	93.90	97.91	7.00	6.10	2.09
Glutamate	3.13	4.18	3.16	3.63	4.26	3.41	101.00	97.10	95.81	1.00	2.90	4.19
GABA	2.76	3.77	3.47	3.70	4.15	4.06	103.50	101.27	93.94	3.50	1.27	6.06

For BH4, Dopamine, Serotonin, norepinephrine, epinephrine, the low, medium, and high control solutions were 30 pg/mg, 500 pg/mg, and 8000 pg/mg of DW, respectively. For glutamate and GABA, those were 6000 pg/mg, 30,000 pg/mg, and 160,000 pg/mg of DW.
For both precision tests, the values were in coefficient of variation (CV).
RE = Relative Error.
Mean of five replicates () observations at each concentration.
^bMean of 25 replicates () observations over five different analytical runs.