Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor

Table 1

Accelerated Solvent Extraction (ASE) conditions for extraction and UFLC attributes during determination of CPT and piperine from N. nimmoniana and P. nigrum, respectively.

Plant name	N. nimmoniana	P. nigrum
Compound	Camptothecin	Piperine
Sample size (g)	1.0	0.1
Extraction solvents	Methanol	Ethanol
Temperature range (°C)	40–80	40–60
Temperature elevation (°C)	10	10
Heat (min)	5	5
Max pressure range (psi)	1525–1675	1520–1570
Static time (min)	1.0	1.0
Static cycles	1.0	1.0
Flush (%)	10	10
Purge time (min)	0.5	0.5
Total extraction time/cycle (min)	6.5	6.5
Linearity equation	𝑦=81036𝑥+41525	𝑦=91335𝑥+28669
𝑅^2	0.992	0.981
LOD (μg mL^−1)	0.2020	0.1173
LOQ (μg mL^−1)	0.6121	0.3554