Comparability of a Three-Dimensional Structure in Biopharmaceuticals Using Spectroscopic Methods

Table 3

Thiol quantification using DLM method. Rituximab and Etanercept free thiols under native and denaturing conditions using DLM method. Dispersion values are presented in SD (𝑛=9).

Protein	Condition	Mabthera	RSD	Kikuzubam	RSD	𝑃
Rituximab	Native	0.0636 ± 0.028	44.1	0.0683 ± 0.033	47.8	0.7499
	Denaturing	1.149 ± 0.480	41.8	1.076 ± 0.508	47.2	0.8027
	Condition	Enbrel	RSD	Infinitam	RSD	𝑃
Etanercept	Native	0.0844 ± 0.017	19.5	0.0477 ± 0.020	41.7	<0.001
	Denaturing	1.091 ± 0.207	19.0	1.067 ± 0.068	6.4	0.7984