Research Article
Comparability of a Three-Dimensional Structure in Biopharmaceuticals Using Spectroscopic Methods
Table 4
Specificity and accuracy of thiol analysis. Controls are specified for DTNB method and DLM method.
| Protein | Condition | Cys/Protein molar ratio | DTNB | DLM |
| Somatropin | Denaturing/reducing | 3.98 ± 0.31 | NT |
| HSA | Native | NT | 1.01 ± 0.17 | Glatiramer acetate | 0.001 ± 0.007 | 0.045a | Placebo Rituximab | 0.0005 ± 0.001 | 0.0004a | Placebo Etanercept | 0.0005 ± 0.001 | 0.0024a | Protein marker | NT | 0.0005a |
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NT: not tested.
aSD value is less than 1 × 10−5.
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