Comparability of a Three-Dimensional Structure in Biopharmaceuticals Using Spectroscopic Methods

Table 5

DyLight Maleimide method and nonspecific interactions tests under native conditions.

Protein	1	2	3	4
	Thiol quantification by DLM method	Nonspecific interaction (DLM neutralization)	Protein amine neutralization (protein isothiocyanate treatment)	Thiol quantification by DTNB Method
A Rituximab	0.0632 ± 0.0274	0.0230 ± 0.0001	0.0426 ± 0.0094	0.0180 ± 0.0032
B Etanercept	0.0965 ± 0.0028	0.0349 ± 0.0000	0.0229 ± 0.0000	0.0240 ± 0.0049
C Glatiramer acetate	0.0450 ± 0.0000	0.0001 ± 0.0001	0.0026 ± 0.0000	0.0010 ± 0.0024