Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics

Figure 1

Peptides and proteins are isolated from body fluids using a fully automated solid-phase extraction protocol based on functionalized magnetic beads. All samples are processed in 96-well plate format with a 96-channel pipetting head, increasing throughput up to 1000 samples per 24 hours. After spotting onto a matrix-assisted laser desorption ionization (MALDI) target plate peptide and protein profiles are acquired on an ultrahigh resolution Fourier transform ion cyclotron resonance (FTICR) system.