Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species

Table 2

Primer information.

Primer name	Gene amplified	Variable region	Primer sequence	Fragment size
V2F [14]	16S	V2	GGCGAACGGGTGAGTAA	239 bp
V2R [14]	16S	V2	ACTGCTGCCTCCCGTAG	239 bp
V3F [15]	16S	V3	CCAGACTCCTACGGGAGGCAG	184 bp
V3R [15]	16S	V3	CGTATTACCGCGGCTGCTG	184 bp
V7F	18S	V7	AACTTAAAGGAATTGACGGAA	156 bp
V7R	18S	V7	GCATCACAGACCTGTTATTGCCCC	156 bp
V9F	18S	V9	GTACACACCGCCCGTCGCACC	310 bp
V9R	18S	V9	TTCCGCAGGTTCACCTACGGA	310 bp

Fragment size estimated from NCBI (http://www.ncbi.nlm.nih.gov).