Research Article

Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF254 for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations

Figure 1

Densitogram of RPHPTLC separation of reference standard of examined nicotinamide (NAM) from its related substances: N,N-diethylnicotinamide (DENAM), N-methylnicotinamide (MNAM), and nicotinic acid (NA) obtained on silica gel 60 RP18WF254 and with the use of methanol-water (3 : 7, v/v) as mobile phase.