Regularities of Anthocyanins Retention in RP HPLC for “Water–Acetonitrile–Phosphoric Acid” Mobile Phases

Table 1

Chromatographic characteristics of 3 glucosides of six common anthocyanidins.

	Solute	Parameters of (3)			, nm
		1	Dp3Glu	1.123 ± 0.010				0.99995	523.0	465.1; 287.0
2	Cy3Glu	1.057 ± 0.008		0.99998	515.0	449.1; 287.0
3	Pt3Glu	1.139 ± 0.011		0.99997	524.0	479.2; 317.0
4	Pg3Glu	1	0	—	500.5	433.2; 271.0
5	Pn3Glu	1.070 ± 0.010	0.136	0.99997	515.0	463.1; 301.0
6	Mv3Glu	1.150 ± 0.010	0.236	0.9999	525.0	493.1; 331.0

^aMobile phase: 10 vol.% CH₃CN and 0.5 vol.% H₃PO₄.