Fast HPLC-MS/MS Method for Determining Penicillin Antibiotics in Infant Formulas Using Molecularly Imprinted Solid-Phase Extraction

Table 4

Maximum recoveries achieved for the different penicillin-based MIP sorbents during MISPE optimization using standard solutions.

MIP^a	Recovery (%)
	AMX	OXA	CLOX	PEN G	PEN V	AMP	DICLOX	NAFC
OXA-MAA-EGDMA-MeOH^b	45	60	28	77	34	50	25	36
AMX-MAA-EGDMA-MeOH^b	15	10	10	6	7	10	7	8
NAFC-MAA-DVB-ACN/TOL^c	27	31	27	22	35	45	22	50
NAFC-MAA-TRIM-ACN^c	25	65	66	54	62	45	58	56
NAFC-MAA-TRIM-MeOH^c	22	82	61	54	65	38	57	51
NAFC-MAA-EGDMA-ACN^c	58	88	82	56	70	65	63	77
NAFC-MAA-EGDMA-MeOH^c	20	58	43	42	56	40	51	49

Polymerization mixture, that is, template-functional monomer-cross-linking monomer-porogen (initiator was always AIMN). ^b,cOptimized extraction conditions for maximum recoveries, elution always with methanol 1% acetic acid; ^bloading: toluene; washing: toluene 10%; ^cloading and washing: acetonitrile.