Fast HPLC-MS/MS Method for Determining Penicillin Antibiotics in Infant Formulas Using Molecularly Imprinted Solid-Phase Extraction

Table 5

Mean recoveries (ten samples), precision, limit of detection (LOD), and limit of quantification (LOQ) obtained for the determination of penicillin drugs in milk powder (infant formula) using NAFC-MAA-EGDMA-ACN polymer as MISPE sorbent, in combination with HPLC-MS/MS.

Parameter^a	Analyte
	AMX	OXA	CLOX	PEN G	PEN V	AMP	DICLOX	NAFC
Recovery (%)	60	84	91	60	60	66	62	74
CVr (%)	9.2	12.1	3.6	5.5	13.9	10.3	1.4	8.1
CVR (%)	10.7	19.2	19.3	9.9	16.0	12.9	2.4	11.1
LOD (µg kg^−1)	0.9	23.6	15.5	0.7	1.2	1.1	1.4	7.4
LOQ (µg kg^−1)	3.1	78.4	51.6	2.4	3.9	3.6	4.8	24.6

CVr: repeatability, greater intraday CV%; CVR: within-lab reproducibility, greater interday CV%.