A Confirmatory Method Based on HPLC-MS/MS for the Detection and Quantification of Residue of Tetracyclines in Nonmedicated Feed

Table 1

Retention time (Rt), cone voltage (CV), collision energy, precursor, and product ions employed for ion identification.

Tetracycline	Rt (min)	Precursor > production	CV	Collision; energy
Tetracycline	12.63	445 > 410	30	29
Tetracycline		445 > 154	30	27
Doxycycline	13.84	445 > 428	30	20
Doxycycline		445 > 125	30	27
Chlortetracycline	11.84	479 > 462	30	23
Chlortetracycline		479 > 444	30	23
Oxytetracycline	12.61	461 > 443	30	20
Oxytetracycline		461 > 426	30	20
Demeclocycline	12.00	465 > 448	30	17