Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin
Table 5
Assay validation sheet of the proposed spectrophotometric methods for the determination of AA and RU.
Parameter
Direct
A_Sum
Iso_RD
A_Sum
Iso_RD
Iso_P
RD
1DD
MCN
RS
RU
RU
RU
AA
AA
AA
AA
AA
AA
AA
Accuracy
(Mean ± SD)
99.91 ± 0.35
96.99 ± 0.55
100.44 ± 1.11
99.66 ± 1.00
101.13 ± 1.42
101.36 ± 1.74
102.10 ± 0.88
101.54 ± 0.24
101.95 ± 0.63
99.46 ± 1.55
Precision
Repeatability
100.28 ± 0.95
95.79 ± 0.63
99.93 ± 1.79
99.93 ± 0.90
99.42 ± 2.02
98.11 ± 1.29
101.36 ± 1.25
99.86 ± 1.29
101.57 ± 1.29
100.49 ± 1.27
Intermediate precision
100.86 ± 2.15
97.91 ± 1.31
98.60 ± 1.32
99.30 ± 1.56
98.09 ± 1.97
99.80 ± 2.17
100.91 ± 1.69
100.75 ± 1.49
100.98 ± 1.32
100.55 ± 1.67
Linearity
Slope
0.0385
−0.0017
0.1416
−0.0017
0.1480
0.5061
0.2850
0.0161
4.6640
0.6381
Intercept
0.0206
0.0042
−0.3615
0.0042
−0.4926
0.0208
−0.0332
0.0332
1.0217
0.0241
Correlation coefficient ()
0.9999
−0.9999
1
−0.9999
0.9952
0.9955
0.9964
0.9964
0.9966
0.9964
Range (μg mL−1)
4–50
4–50
4–50
4–50
4–50
4–50
4–50
4–50
4–50
4–50
LOQ (μg mL−1)
1.13
1.39
0.49
1.39
1.54
3.04
1.20
0.42
1.04
2.70
LOD (μg mL−1)
0.37
0.46
0.16
0.46
0.51
1.00
0.40
1.28
0.34
0.89
The intraday (), average of three concentrations (12, 16, and 20 μg mL−1) for AA and RU repeated three times within the day. The interday (), average of three concentrations (12, 16, and 20 μg mL−1) for AA and RU repeated three times in three successive days.
