Quality Control of 11 Cannabinoids by Ultraperformance Liquid Chromatography Coupled with Mass Spectrometry (UPLC-MS/MS)

Table 1

Statistical analysis of a six-point calibration curve from 1 ng/mL to 200 ng/mL, with five replicates for calibration standards and quality control (QC) standards for the 11 cannabinoid mixture. Tandem mass spectrometry (MS/MS) transitions calibration range results for potency. The table shows the quantitative abilities of this method for each analyte.

Names	Retention time (min)	MS/MS ion transitions	Concentration ranges (ng/mL)	Linearity (R^2)	LOD	LOQ
Δ^9-THCA	6.4	357.2101 ➔ 313.2145	0–200	0.990	10	25
CBDA	4.3	357.2066 ➔ 245.1538	0–200	0.995	5	10
CBGA	4.5	359.2192 ➔ 341.2126	0–200	0.992	5	10
CBG	4.5	317.2470 ➔ 193.1223	0–200	0.993	10	50
CBD	4.6	315.2336 ➔ 259.1668	0–200	0.996	10	25
THCV	4.5	287.2031 ➔ 165.0929	0–200	0.998	10	25
CBN	5.3	311.2011 ➔ 223.1130	0–200	0.997	5	10
Δ^8-THC	5.91	315.2336 ➔ 193.1242	0–200	0.993	5	10
Δ^9-THC	5.8	315.2324 ➔ 259.1705	0–200	0.992	10	25
CBC	6.4	315.2336 ➔ 193.1242	0–200	0.991	25	50
CBDV	3.4	287.19771 ➔ 65.0892	0–200	0.992	5	10

LOD, limit of detection; LOQ, limit of quantification. Values of linearity refer to the linear range.