Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow Strip

Figure 1

Principle of the multiplex strip for sensing KPC and NDM genes based on barcode capture probes and asymmetrical PCR. (a) Construction of the asymmetric PCR system for amplifying KPC-2 and NDM-1. (b) Design and configuration of the barcode colloid gold strip based on asymmetric PCR. (c) Binding and colour development signal of KPC and NDM genes (positive test). (d) Binding and colour development signal in the absence of KPC and NDM genes (negative test). (e) Interpretation of reagents and probes used in the strip and asymmetrical PCR system. (f) Appearance of the control line demonstrated valid test results; appearance of one or two test lines indicated a positive result for the corresponding carbapenemase encoding genes; no test line indicated a negative result for the two carbapenemase gene; the disappearance of the control line demonstrated invalid test results.