Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow Strip

Figure 4

Optimization of annealing temperature for asymmetric PCR. (a) Asymmetric PCR biotin-ssDNA products for KPC-2 and (b) NDM-1 at varied annealing temperatures by agarose gel electrophoresis. (c) Visual signal of the lateral strip for KPC-2 and NDM-1 at different annealing temperatures and (d) the corresponding intensity detected by using the GIC-H1 portable analyser.

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