Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow Strip

<div>Optimization of the ratio of forward to reverse primer for asymmetric PCR. (a) Biotin-ssDNA products for KPC-2 and (b) NDM-1 at varied <i>F</i>/<i>R</i> ratios analysed by gel electrophoresis. (c) Visual signal of the lateral strip for KPC-2 and NDM-1 at varied <i>F</i>/<i>R</i> ratios and (d) the corresponding intensity detected by using the GIC-H1 portable analyser.</div>

Journal of Analytical Methods in Chemistry

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Figure 5

Figure 5: Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow Strip 