Research Article
Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow Strip
Figure 6
Optimization of the cycle number for asymmetric PCR. (a) Biotin-ssDNA products for KPC-2 and (b) NDM-1 at varied cycle numbers analysed by agarose gel electrophoresis. (c) Visual signal of the lateral strip for KPC-2 and NDM-1 at varied cycle numbers and (d) the corresponding intensity detected by using the GIC-H1 portable analyser.
(a) |
(b) |
(c) |
(d) |